HansaBioMed Life Sciences

Application Notes

Technical articles, methods and case studies. 22 articles.

FLuoEVs as a Robust Positive Control for EV Uptake Assays
FLuoEVs: Purified EVs expressing EGFP (lyophilized)

FLuoEVs as a Robust Positive Control for EV Uptake Assays

Stably fluorescent and non-bleaching FLuoEVs act as valuable positive control materials during cell uptake assays.

Fluorescent EVs from HEK293 cells (CD63-EGFP) (100µL vial)
How to Get Rid of Background Fluorescent Signal: miniPURE EVs Spin for Unbound Dye Removal
Size Exclusion Chromatography Columns

How to Get Rid of Background Fluorescent Signal: miniPURE EVs Spin for Unbound Dye Removal

The versatility of our spin centrifugal columns apply to labeling workflows: You can utilize them to minimize background fluorescent signal by removing unbound dyes.

miniPURE-EVs Spin: Centrifugal Size Exclusion Chromatography Columns
Tangential Flow Filtration (TFF) Provides an Easy and Robust Method for the Isolation of Urine EVs
TFF Cartridges and Concentrators

Tangential Flow Filtration (TFF) Provides an Easy and Robust Method for the Isolation of Urine EVs

TFF cartridges can be used in combination to isolate EVs from urine with high yield and purity, while minimizing abundant proteins like THP.

Beyond First Impression: The Reusability of TFF Cartridges
TFF Cartridges and Concentrators

Beyond First Impression: The Reusability of TFF Cartridges

TFF cartridges can be used multiple times without significant deterioration in their yield and purity performance metrics.

Reliable Single-EV Analysis: The PICO Assay with TetraEVs Standards for High-Reproducibility Research
Engineered EVs

Reliable Single-EV Analysis: The PICO Assay with TetraEVs Standards for High-Reproducibility Research

TetraEVs are valuable standards for validating and calibrating high-sensitivity single EV quantification assays.

Engineered EVs from HEK293 cells overexpressing CD63 (100µL vial)
Less Lipoproteins, Higher Purity: Why You Should Consider Platelet EVs over Plasma EVs
Exosomes and EVs

Less Lipoproteins, Higher Purity: Why You Should Consider Platelet EVs over Plasma EVs

Platelet EVs are an attractive alternative to study plasma EVs with much higher purity from lipoproteins.

Lyophilized and purified exosomes from platelets of healthy donors
Working with Primary Cancers: TFF+SEC for High-Efficiency EV Isolation
TFF Cartridges and Concentrators

Working with Primary Cancers: TFF+SEC for High-Efficiency EV Isolation

TFF cartridges in combination with SEC columns offer a suitable solution for high efficiency EV isolation from primary cancer media.

Platelet EVs: A New Reference Material from Blood Components with Superior Purity over Plasma EVs​
Exosomes and EVs

Platelet EVs: A New Reference Material from Blood Components with Superior Purity over Plasma EVs​

As blood EV components free of lipoproteins, platelet EVs offer superiority over their plasma counterparts.

Lyophilized and purified exosomes from platelets of healthy donors
Tiny Packages, Big Impact: Functional Evaluation of Lyophilized Platelet and MSC-EVs
Exosomes and EVs

Tiny Packages, Big Impact: Functional Evaluation of Lyophilized Platelet and MSC-EVs

Lyophilized platelet and MSC-EVs are useful for benchmarking for functional assays including wound healing and angiogenesis.

Lyophilized and purified exosomes from platelets of healthy donors
Going Beyond Eukaryotes: Purification of Bacterial EVs
Exosomes and EVs

Going Beyond Eukaryotes: Purification of Bacterial EVs

Our TFF cartridges are useful not only for eukaryotic cells, but also for prokaryotic cells for high-yield EV isolation.

Lyophilized Bacterial EVs Derived from Lactobacillus (100µg vial)
EV Concentration the Other Way: Reverse Ultrafiltration over V-Shaped Filters
EV-Spinner

EV Concentration the Other Way: Reverse Ultrafiltration over V-Shaped Filters

Reverse ultrafiltration by EV-Spinner ensures superior purity and yield over V-shaped filters during reuse.

NanoSorter® Meets FLuoEVs: Precision Sorting of Extracellular Vesicles
FLuoEVs: Purified EVs expressing mCherry (lyophilized)

NanoSorter® Meets FLuoEVs: Precision Sorting of Extracellular Vesicles

Double-labeled FLuoEVs offer valuable reference materials for multi-channel fluorescent measurements.

Fluorescent EVs from HEK293 cells (double labeled with EGFP and mCherry) (100µL vial)
Tangential Flow Filtration for Efficient Depletion of Nanoparticles Contained in Fetal Bovine Serum
TFF Cartridges and Concentrators

Tangential Flow Filtration for Efficient Depletion of Nanoparticles Contained in Fetal Bovine Serum

TFF-EVs can efficiently deplete FBS of nanoparticles.

TFF-EVs Small: Small EV/exosome purification
Comparative Evaluation of EV Isolation Methods: Tangential Flow Filtration vs Ultracentrifugation
TFF Cartridges and Concentrators

Comparative Evaluation of EV Isolation Methods: Tangential Flow Filtration vs Ultracentrifugation

TFF offers various advantages over conventional ultracentrifugation method for EV isolation.

From Sample Preparation to Data Analysis: A Comprehensive Workflow for Imaging EVs with SMLM
FLuoEVs: Purified EVs expressing EGFP (lyophilized)

From Sample Preparation to Data Analysis: A Comprehensive Workflow for Imaging EVs with SMLM

FLuoEVs act as useful positive controls for super-resolution microscopy workflows.

Fluorescent EVs from HEK293 cells (CD63-EGFP) (100µL vial)
Assessment of Extracellular Vesicle Corona with ExoTEST ELISA and MP-SPR
Exo-TEST: Ready to use ELISA Quantification Kits

Assessment of Extracellular Vesicle Corona with ExoTEST ELISA and MP-SPR

ExoTest can be utilized as a tool for EV corona studies.

ExoTEST Ready to Use test Kit for Overall Exosome capture and quantification from cell culture supernatant
Digging Deeper into Nanoscale: Size-Based Extracellular Vesicle Characterization with Taylor Dispersion Analysis
FLuoEVs: Purified EVs expressing EGFP (lyophilized)

Digging Deeper into Nanoscale: Size-Based Extracellular Vesicle Characterization with Taylor Dispersion Analysis

FLuoEVs are ideal reference materials for fluorescence-based approaches for EV quantification.

Fluorescent EVs from HEK293 cells (CD63-EGFP) (100µL vial)
Sequential Tangential Flow Filtration for Purification of Plant-Derived Nanoparticles
TFF Cartridges and Concentrators

Sequential Tangential Flow Filtration for Purification of Plant-Derived Nanoparticles

TFF is an ideal method for scalable, high-throughput and high-purity isolation of EVs from various plant sources.

miniPURE-EVs Spin Column: A New Platform for a Fast and Convenient Purification of EVs and Nanoparticles from Small Volume Amount
Size Exclusion Chromatography Columns

miniPURE-EVs Spin Column: A New Platform for a Fast and Convenient Purification of EVs and Nanoparticles from Small Volume Amount

Spin columns are versatile solutions for EV purification from minimal sample volumes or removal of unbound dyes and antibodies.

miniPURE-EVs Spin: Centrifugal Size Exclusion Chromatography Columns
Human Platelet-derived Extracellular Vesicles (EVs): A new EVs reference Material from Blood Components
Exosomes and EVs

Human Platelet-derived Extracellular Vesicles (EVs): A new EVs reference Material from Blood Components

Our method of isolation of platelet EVs ensure scalable manufacturing, reproducibility, and a consistent lipoprotein reduction.

Lyophilized and purified exosomes from platelets of healthy donors
Optimized Tools and Protocols for Fast and Standardized Fluorescent Analysis of EVs
TetraEVs: Purified EVs overexpressing CD63 (lyophilized)

Optimized Tools and Protocols for Fast and Standardized Fluorescent Analysis of EVs

TetraEVs and fluorescent beads by HBM prove useful for optimizing workflows for fluorescent EV labeling.

A Novel Tool for Efficient RNA Loading into Extracellular Vesicles: Advancing Gene Therapy
Exosomes and EVs

A Novel Tool for Efficient RNA Loading into Extracellular Vesicles: Advancing Gene Therapy

Lyophilized EV reference materials by HBM are useful benchmarks for drug delivery research.