Working with Plant EVs
Plant EVs, called also plant EV-like nanoparticles (ELNs), are attracting interest from researchers in academic sector, but also from the nutraceutical and the cosmetic industry. Indeed, plant EVs, such as the EV produced by mammalian cells, reflect the properties of the parental cells, can be purified from the plant leaves, stems, or juice, easily sterilized by filtration, and long-term stored. We started studying and working with extracellular vesicles from plant two years ago, in the context of a research project aimed to evaluate the particles produced by plants, in particular from Estonian origin, as potential source of regenerative and protective properties for skincare. In this period, we have developed expertise and methods for the purification and the characterization of vesicles from different plants, including rhizomes (ginger, ginseng), tubers (potato), Allioideae (garlic, onion), Cruciferae (cabbage family) and plant juices (coconut water, pineapple juice, orange and lemon juice). Our current workflow includes the preparation of the plant extract by plant juicer, the EV purification by Tangential Flow Filtration (TFF) or combination of TFF and Size Exclusion Chromatography. Among the particle analysis we can perform NTA, phenotyping by detecting the marker TET8, morphology analysis by Transmission Electron Microscopy.
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