Sample type:

Urine, conditioned cell media or other diluted matrices

Quantity:

1 maxi column + 1 TFF-MV concentrator + 1 TFF-Easy concentrator

Product overview:

EVs can be effectively separated using a combination of double tangential flow filtration (TFF) and size exclusion chromatography (SEC).

TFF-MV allows to separate large microvesicles (>150 nm) from smaller vesicles. TFF-Easy allows to concentrate the remained matrix prior to EV isolation.

SEC column allows to isolate EVs from small molecules and ions in a fast and highly efficient way.

As a result, you get extracellular vesicles of a high purity in less than one hour.

Volume of sample:

5 ml – 20 ml (concentrated 10 folds by TFF-Easy)

Shipment and storage:

4°C.

Shelf life:

12 months

Applications:

Extracellular vesicle isolation from diluted matrices (urine and cell culture media).

Purification of pre-isolated EVs from contaminants.

Sterility:

Not sterile