FLUO-EVs are stably-fluorescent Extracellular vesicles expressing the fluorescent protein EGFP (green) as fusion protein with tetraspanins CD9, CD81 and CD63. FLUO-EVs are purified in combination of Tangential Flow Filtration (TFF) and Size Exclusion Chromatography (SEC) from genetically engineered cells in order to express the fluorescent proteins as fusion protein with the tetraspanin CD9, CD63 or CD81. FLUO-EVs demonstrated high stability of the fluorophores, they can be used for vitro tracking studies or as reference material for analyzers of nanoparticles or for assay calibration.

Characteristics: Particle size distribution 50 – 140 nm, lyophilized, storage at 4-8 °C.

Applications: Positive control for EV analyzers (NTA, flow Cytometers, fluorescence microscopy), cell spike-in and in vitro tracking, EV uptake monitoring, assay and instrument calibration.