Size Exclusion Chromatography (SEC) is considered one of the best methods for isolating and purifying Extracellular Vesicles (EVs) from different matrices. The method is fast, preserves the original shape and functionality of the vesicles, and removes the most of contaminants and circulating proteins.

The miniPURE-EVs-Spin is a new SEC spin column designed for isolating EVs in a fast and easy way from small volume amount ( 0.02 – 0.2 ml).  miniPURE-EVs Spin column is suitable for EV isolation from multiple fluids, including cell conditioned media, plasma, serum, urine. Moreover the column is an optimal platform for the removal of unbound dye post particle labelling, or removal of the excess of fluorophore conjugated antibodies.

The column is compatible with both fixed angle and swinging-bucket centrifuge rotors.

Applications: EV isolation from biofluids and cell media. Removal of unbound dye post EV labelling. Working volume range: 0.02 – 0.2 ml.

Advantages: Easy and fast protocol (turnaround time approximately 5 minutes). No fraction collection. Reusable up to 3 times. Easy to store and ship (4°C).

Mini-PURE-EVs Spin tech note.