Scalable EV purification from biofluids, conditioned media, plants.
Method: Tangential flow filtration and size exclusion chromatography.
Separation of small (40 - 150 nm) and large (>150 nm) EVs.
Alternative methods: ultracentrifugation, immunoaffinity.
Validation: NTA (Zetaview, Particle Metrix), protein marker expression, total protein content.
Characterization of the EV morphology:
Transmission Electron Microscopy (TEM).
Immuno Electron Microscopy.
Characterization of EVs by the following methods:
ELISA high throughput marker screening, ideal for biomarker discovery.
Flow Cytometry: characterization by NanoFCM analyzer.
Fluorescence NTA: characterization by Zetaview analyzer (Particle Metrix).
Proteomic and trascriptomic analysis of EV markers:
Mass spectrometry: Sample preparation and Q Exactive Plus 2h nano-LC/MS/MS analysis with MaxQuant-based identification and quantitation.
RNA seq: Library preparation with Nextflex kit. Sequencing done by Illumina Nextseq 500
HansaBioMed Life Sciences not only commercializes products for EV research, but also actively contributes to the development of new technologies for the isolation, characterization and study of exosomes and extracellular vesicles.
Discover our research program
