HANSABIOMED LIFE SCIENCES (HBM LS) IS PARTICIPATING IN THE FOLLOWING PROJECTS
funded under H2020 from European Commission:
Coordinator: CONSIGLIO NAZIONALE DELLE RICERCHE
MARVEL out-of-the-box solution is a paradigm shift from antibodies to peptides as an alternative class of affinity ligands characterized by high efficiency of EV capturing (Figure 2). In particular, MARVEL will introduce the use of membrane-sensing peptides (MSP) as novel ligands for the size-selective capturing of small EV, unbiased by differential surface protein expression (membrane as universal EV marker); in parallel, specific peptide probes (SPP) with high affinity for clinically relevant EV protein markers will be developed to enrich selective EV subpopulations. The versatility and modularity in scaling-up of the technology will be demonstrated on medium/large sample volumes in:
1) the manufacturing of GMP-compliant EV as a medicinal product for cardiac repair;
2) laboratory scale urine-based liquid biopsy for bladder cancer stratification and monitoring.
Program name: FET
Coordinator: Paolo Bergese, CSGI
The main objective of BOW is to create hybrid magnetic nanoparticles with a membrane surface made from an extracellular vesicle.
Within the BOW project HBM will participate in activities aimed at optimization of EV production, providing components such as EV depleted media, labelling reagents and protocols, and EV standards, as well as portable size focusing devices for SEC and TFF; Our team will contribute to comprehensive EV, and evMBDs characterization, and will ultimately verify and define minimal analytical package for definition of EV purity and quality (integrity, stability, and activity) to be assessed at each step of EV production scale up and engineering. Finally, HBM will perform in vitro tropism testing preceding definition of administration protocols for in vivo testing (doses and timeline).
Program name: ITN MSCA
Coordinator: Lorraine O'Driscoll, Trinity College Dublin
TRAINING in EXTRACELLULAR VESICLES: for benefit in Health and Disease HBM LS will evaluate and implement Costefficient high-throughput isolation methods; methods for characterizing EVs, applying bioinformatics analysis to extract biological meaning.
Program name: FET
Coordinator: Marcella Chiari, CNR Italy, Milano
Integrated nanoparticle isolation and detection system for complete on-chip analysis of exosomes feasibility study on that extraction and analysis of exosomes from plasma can be fully integrated and automatized, giving standardized and reliable quantitative readouts
Program name: FET Open
Coordinator: Paolo Bergese, Brescia University
The Extracellular Vesicle Foundry Isolation and QC of high yield, high quality, QC incorporated bioprocess for EVs production in human use.