PURIFICATION OF LARGE AND SMALL EVs

HansaBioMed Life Sciences provides high expertise for purification of Large (>150 nm) and Small EVs (30-150 nm) from human or animal biofluids, cell conditioned media and plant extract. EV purification can be obtained following our standard protocols which combine Tangential Flow Filtration (TFF) and Size Exclusion Chromatography (SEC). Alternatively, differential ultracentrifugation and microfiltration, chemical precipitation or immunoaffinity methods can be used.

Isolated EVs are subsequently quantify for total protein contents and validated by nanoparticles tracking analysis (NTA) with Zetaview (Particle Metrix). Additional characterization can be performed by ELISA, FACS, TEM, Western blotting, on request. Purified EVs can be shipped to the customer lyophilized or frozen, as required.

 

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